Signifikanta korrelationer har påvisats mellan dräktighetsprocent och viabilitet hos propidium- iodid färgad sperma som analyserats med en flowcytometer. Signifikanta, men låga korrelationer har kunnat påvisas mellan fölningsprocent och spermiernas subjektiva motilitet efter en inkubationstid på 2 och 4 timmars vid 37☌ samt med en hypo-osmotisk svällningstest efter 0 och 3 timmars inkubation. Det är svårt och dyrt att inseminera tillräckligt många ston för att erhålla statistiskt signifikanta resultat. Få studier har gjorts för att undersökakorrelationen mellan fertiliteten hos fryst hingst sperma och laboratorietest. Metodernas begränsningar, det praktiska utförandetoch korrelationen med fertilitetsresultaten diskuteras. En översikt.ĭenna översiktsartikel refererar olika in vitro undersökningsmetoder som använts på frys-tinad hingst sperma. Computer assisted automatic sperm analyzers have replaced light microscopy in research projects, but so far nobody has been able to demonstrate a correlation between fertility of frozen stallion semen and any of the motility parameters obtained by these instruments.īedömning av frys-tinad hingst sperma med hjälp avin vitro metoder. In spite of that, motility estimation by light microscope is the most commonly used method to evaluate frozen-thawed stallion sperm. No correlations have been detected between fertility and motility immediately after thawing. Significant correlations have been reported between the pregnancy rate and viability of propidium iodide-stained sperm assessed by flow cytometry as well as for glass wool and Sephadex filtration tests. Significant, but low correlations have been demonstrated between the foaling rate and subjective motility of sperm incubated for 2 h and 4 h at 37☌ and hypoosmotic swelling test after 0 and 3 h of incubation. It is difficult and expensive to inseminate an adequate number of mares to achieve statistically significant differences. Very few studies have tried to find correlation between fertility of frozen stallion semen and laboratory tests. The techniques, limitations of the methods and correlations with fertility results are discussed. The article reviews methods used for in vitro evaluation of sperm, with particular emphasis on frozen-thawed stallion sperm.
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